Composite

Part:BBa_K5317008:Design

Designed by: Jan Gelhoet   Group: iGEM24_Hannover   (2024-09-13)


MREwt promoter-EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was important to consider the MRE site positioning for optimal MTF-1 and MRE site interaction and not disturb the start and stop codon as well as avoid frame shifts of the EGFP sequence during cloning.


Source

The MREwt promoter was synthesized and the EGFP gene was available from the EGFP-C2 backbone (K3338020).

References